Regulatory

Part:BBa_K309003:Design

Designed by: Chris Palmer   Group: iGEM10_Queens-Canada   (2010-10-22)


pStr-1 (C. elegans promoter)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1022
    Illegal XbaI site found at 1028
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1025
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1022
    Illegal XbaI site found at 1028
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1022
    Illegal XbaI site found at 1028
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The exact length and sequence of the functional sequence that acts as the promoter in C. elegans has not been elucidated. Thus this sequence is a section of genomic DNA that exists in front of the Gpd-2 gene that has been previously demonstrated to function as a promoter, with the same expression pattern as the Gpd-2 gene.


Source

From C. elegans genomic DNA.

References